jQuery UI Menu - Default functionality
RSS

Molecular Biology


Fragment Analyzer                                                                                                                                                                                           

Fragment Analyzer

Dramatically simplify life in your lab with an automated workflow that brings you the best in productivity and precision.

Take a few seconds to set it up and Fragment Analyzer will automate capillary electrophoresis—giving you the power to optimize runs for speed or resolution.

Innovative design features ensure you get gold-standard data, while the one-step setup reduces the risk of handling errors creeping into your results, as it can with manual agarose and lab-on-a-chip methodologies. Capillaries are auto-flushed between runs to eliminate cross contamination and further underline data integrity.

When it comes time to view results, our software processes the data and visualizes it in your preferred format. All you need to do is choose the kit for your application, set the instrument and load your samples—from 12 to 288 at once.

"The Fragment Analyzer provides us with superior data compared to our previous methods…"

Dr. Henry Long, Dana Farber Cancer Institute.
Read More

  • Run 12- or 96-capillary plates
  • Resolves fragments from 10 bp to 40,000 bp
  • Get resolution down to 2 bp for fragments
  • Detection starts at 5 pg/μL

Fragment Analyzer is used and recommended by Illumina™ as an automated system for the quantification and qualification of NGS libraries, gDNA, and RNA. Illumina elaborates on its website, mentioning Fragment Analyzer as one of the answers to “laborious and time-consuming” constraints in the lab while making it “easier for our customers” to automate fragmentation and size selection.

ZAG

ZAG DNA Analyzer

Slab gel electrophoresis methods are time-consuming and prone to error. Now there’s a new method that will quickly and accurately run your DNA samples. 

Simple sample setup The ZAG DNA Analyzer holds up to nine 96-well deep plates. Unlike slab gels or microfluidic chips, you can quickly and easily fill the plates with a multichannel pipetter. ZAG also holds two different separation gels which allow you to set up varying runs ahead of time. Simply set it up and walk away. ZAG will automatically switch to the gel you specified when setting up the run. Both capabilities increase the efficiency of your lab.

Even faster automation With a simple modification, your ZAG can be set up for automation using a robot to change out the good plates.

Fast separations When analyzing DNA fragments with the ZAG DNA Analyzer, a 96-well plate of direct PCR or diluted amplicon sample can be ready for assessment in as little as 20 minutes.

High sample capacity When coupled with the ZAG-135 reagent kit, you’ll experience the highest throughput of any platform offered on the market. The ZAG DNA Analyzer uses a 96-capillary array that runs simultaneous injection of all 96 samples. A similar AATI design was a major reason the company won the Frost and Sullivan 2014 New Product Innovation Award.

LOW COST per sample ZAG competes for cost-wise vs. agarose after adding in the total cost of running gels. Factor in every step associated with time and labor for traditional manual gels: 

    • Weighing the agarose
    • Adding the buffer
    • Heating to melt the agarose
    • Preparing the gel plate and comb
    • Pouring the molten agarose
    • Waiting for the agarose to solidify
    • Preparing the running buffer
    • Removing the comb and placing gel into the electrophoresis chamber
    • Loading the DNA samples
    • Waiting for separation to occur
    • Staining the nucleic acids
    • Photographing the separations
    • Interpreting the bands

Next, factor in lost time related to human error or differences in slightly varying techniques from technician to technician – because lab workers will sometimes “call-out” the bands differently. Finally, slab gels require technical staff to work with buffers containing toxic chemicals and dyes.

ZAG eliminates all of these problems.

The benefits of using the ZAG DNA Analyzer as compared to agarose slab gels are:

  • No more pouring gels. Simultaneous analysis of 12 to 96 samples giving you analysis of up to 864 samples automatically.
  • Higher sensitivity than agarose gels. Use small DNA sample amounts (0.1 ng).
  • Ultra fast lower marker (set to 1bp) migrates faster for superior quality and quantity assessment.
  • Good sizing capability to differentiate degraded, partially degraded or intact DNA.

With low sample cost, the ZAG DNA Analyzer is the most affordable DNA analysis instrument on the market.

Our patented technology start to finish has a significant impact on your data output including:

  • High-throughput analysis with virtually no failure rate.
  • Built-in PROSize® 2.0 Software will process over 100 sample plates at once and allow for batch processing.
  • Automatically integrates your data set into a LIMS, .csv or .pdf format.

Fragment Analyzer FEMTO Pulse

    

Fragment Analyzer FEMTO Pulse

Advances in molecular biology are driving life science research forward at an unprecedented rate. Progress is often inhibited by slow and outdated methods of analysis that have not kept up with this breakneck speed. The FEMTO Pulse is a revolutionary instrument for the analysis of nucleic acids. Capable of detecting down to 1 fg/µl of the sample and accurately sizing fragments and smears through 165,000 bp, the FEMTO Pulse is breaking all the QC barriers.

The enhanced sizing capability of the FEMTO Pulse is the direct result of incorporating a pulsed-field power supply into the instrument. Pulsed-field electrophoresis is a powerful separation method and a proven way to separate very large DNA fragments and smears. On the FEMTO Pulse, three factors are controlled to provide high-quality separations of large nucleic acid fragments and smears:

  • Voltage polarity
  • Oscillation frequency
  • Wave form of the oscillation frequency

The FEMTO Pulse has all the separation advantages of pulsed-field electrophoresis but is able to complete a separation 15-20X faster than the current method while automating the analysis.

Figure 1. A large fragment ladder separation on the FEMTO Pulse.

The high sensitivity of the FEMTO Pulse is the result of a combination of several factors, including:

  • Optimized optical sensors
  • Improved illumination and excitation at the detection windows of the intercalation dye
  • Redesigned algorithms that facilitate improved detection
  • Improved gel chemistries

Figure 2. Dilution series showing the 1 fg/ul sensitivity of the FEMTO Pulse.

The combination of femtogram level sensitivity and accurate sizing of very large nucleic acid fragments and smears allows the FEMTO Pulse to break analytic barriers.